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I am looking for a freely distributed dataset of microscopic images. I am hoping to find a dataset that would feature histological images before and after staining (e.g. H&E or some other standard one, preferably). I have been searching through various databases, but I cannot find a proper set. I hope the sample size to be at least about 100. Suggestions?

user8640
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  • What do you mean, "before and after staining"? An image taken before staining won't show anything... – MattDMo Aug 06 '14 at 18:45
  • Oh, I must have understood something incorrectly. Well, I hope I can have access to the "raw" data, as well as the processed versions. – user8640 Aug 06 '14 at 18:49
  • There should be very minimal processing for scientific images - otherwise you are not looking at true results. – MattDMo Aug 06 '14 at 18:51
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    Do you have a particular species in mind? are you looking for something like this? (http://www.virtualflybrain.org/site/stacks/index.htm) – Behzad Rowshanravan Aug 07 '14 at 00:52

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Virtual fly brain (http://www.virtualflybrain.org/site/stacks/index.htm) is an excellent database of fly (Drosophila) brain sections, which can be electronically processed/annotated/highlighted on the fly to show different areas of the fly brain as an overlay on top of the original images.

Alternatively, you can use BrainTrap (http://fruitfly.inf.ed.ac.uk/braintrap/) for the expression of proteins in the fly (Drosophila) brain using brain sections. Another database which has silver stains of Drosophila brain sections is the flybrain (http://flybrain.neurobio.arizona.edu/Flybrain/html/atlas/) database. This database also contains 3D models of different fly brain structures and much more!

I'm sure there are many many similar databases, but these are the ones I knew of, which are widely used in the Drosophila community.

Behzad Rowshanravan
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