The scientific study of the structure and function of genes at the molecular level, particularly chromosomes and DNA.
The scientific study of the structure and function of genes at the molecular level, particularly chromosomes and DNA.
Questions tagged [molecular-genetics]
675 questions
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Can translation ever occur without transcription?
This question is aimed mainly towards viruses with ssRNA, is transcription required, for the +ve strand RNA? If so, why?
user37985
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How does DNA codon (5`-3`) convert to mRNA?
There is a problem I stumbled upon when doing a lesson on molecular genetics (Grade 12 level).
The lesson contains many tables in this format:
I don't understand how it got to the mRNA codon considering how RNA polymerase creates mRNA. From what I…
ReftPowered
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What does z in lac z, y in lac y and a in lac a gene stand for?
In lac operon the 3 structural genes lacz, lacy and laca must have some reason behind their names. While lac refers to lactose but what does z, y and a refer to?
user54844
3
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1 answer
When DNA is at its uncondensed form, what can it do?
I think it can do two things:
The cell may be duplicating the genome during S phase.
The cell may be transcribing the DNA into mRNA.
Question: Can the two activities occur at the same time or one at a time? In other words, are cells efficient…
Double U
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If post transcriptional modification happens , how can a gene code for amino acids in a particular order?
Post transcription, introns are removed and exons are rearranged randomly. If that's the case (which it is according to the book EDEXCEL SNAB A2 topic 6) then shouldn't the protein produced be different every time? Shouldn't the order of amino acids…
Lia Ahmed
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PCR processes number of amplicons produced
Beginning with 600 template DNA molecules after 25 cycles of PCR how many amplicons will be produced?
Rajani Raju
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Question about mutation of multiple nuclei?
If an organism with multiple nuclei, such as the slime mold Physarum polycephalum, was to be exposed to a mutagen, such as 5-bromouracil, what would be the effect (besides death)? Especially if multiple nuclei were effected differently by the…
alotofquestions
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what is the function of satellite in a chromosome?
What is the specific function of a satellite in a chromosome ? How are sat-chromosomes different from others ? On molecular basis, what are the functional advantages of it ?
Captain Curious
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How is a stem-cell induced to assimilate a knock-in DNA construct?
While looking at (daylight) color perception I came across a paper by Jeremy Nathans (et al., Proc. Nat. Acad. Sci., vol. 100 no. 20, 2003, full text here) in which he describes the creation of a mouse with genetically modified vision.
My threshold…
daniel
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Use of restriction endonucleases to analyse Variable Number Tandem Repeats (VNTRs)
The textbook I’m using states that “Ends of VNTR have conserved sequences. These can easily be cut by restriction endonucleases.”
But restriction endonucleases cleave palindromic sequences. Does this mean that the repeated DNA and conserved…
A_Sheth
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How do homologous pairs find each other
If I'm not mistaken the only time homologous pairs of chromosomes need to find each other is during gamete formation in preparation for crossover recombination.
How do they find each other?
user55481
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What exacly happens at chromossome level during fecudation and in the first zygotic division?
I was reading about the viability of animal hybrids and found the following sentence here:
Previously, this idea was not widely accepted, because, in general, different species have different numbers of chromosomes, the structures within cells that…
Natanael Antonioli
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Why is trisomy a problem?
I understand that a missing part of genetic information is a problem - the cell does not know how to synthesize a specific protein. But why is it a problem when there's one more copy of a chromosome?
Let's say a trisomy of the 21st chromosome…
Deritus
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Loss in DNA band size extracted from polyacrylamide gel
I'm using cDNA AFLP teq.. I extracted 670 bp band from polyacrylamide (urea) gel and I have amplified with PCR but on agarose gel it stops at nearly 350 bp.it happened for most of other bands before. I always extracted the precise bands. I…
sh.v
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Transduction with P1 lysate prepared from strain that is proC::Tn10
If I do a transduction with a P1 lysate prepared from a strain that is pro*C*::Tn10 and the recipient is wild type, will all my recombinants be ProC- and Tet resistant since the Tn10 is in the proC gene?
user4942
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