Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

Some Basic Definitions:

Biochemistry is the branch of science that explores the chemical processes within and related to living organisms. It is a laboratory-based science that brings together biology and chemistry. By using chemical knowledge and techniques, biochemists can understand and solve biological problems.(1)

Biochemistry is the study of the chemical substances and vital processes occurring in living organisms. Biochemists focus heavily on the role, function, and structure of biomolecules. The study of the chemistry behind biological processes and the synthesis of biologically active molecules are examples of biochemistry.(2)

Brief introduction:

History:

The history of biochemistry can be said to have started with the ancient Greeks who were interested in the composition and processes of life, although biochemistry as a specific scientific discipline has its beginning around the early 19th century. Some argued that the beginning of biochemistry may have been the discovery of the first enzyme, diastase (today called amylase), in 1833 by Anselme Payen, while others considered Eduard Buchner's first demonstration of a complex biochemical process alcoholic fermentation in cell-free extracts to be the birth of biochemistry. Some might also point to the influential work of Justus von Liebig from 1842, Animal chemistry, or, Organic chemistry in its applications to physiology and pathology, which presented a chemical theory of metabolism, or even earlier to the 18th century studies on fermentation and respiration by Antoine Lavoisier.

Main branches:

  • Animal Biochemistry (biochemistry of animals)

  • Plant Biochemistry (biochemistry of plants)

  • Molecular Biology (concerns the molecular basis of biological activity between biomolecules in the various systems of a cell)

  • Cell Biology (study of the different structures and functions of the cell)

  • Metabolism (conversion of food/fuel to energy to run cellular processes, conversion of food/fuel to building blocks for macromolecules, and the elimination of nitrogenous wastes)

  • Immunology (study of immune systems in all organisms)

  • Genetics (study of genes, genetic variation, and heredity in living organisms)

  • Enzymology (study of enzymes i.e. macromolecular biological catalysts)

Applications:

When to use the biochemistry tag?

As defined, this tag should be used for questions regarding chemistry within the scope of biology: the compounds involving/related to cellular metabolism, cellular biology, genetics, biomolecules, enzymes, immune system and the reactions involving them in living organisms.

Links for further information:

2013 questions
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What is the most condensed form of (stored) energy used in biology?

When considering how organisms store energy for later use, I was wondering what substance known in Biology is best for compacting the most energy in the smallest space(volume-wise). With my (limited) knowledge, I would guess that it would be what we…
DoubleDouble
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How fast will cells lyse under hypotonic conditions?

Routinely, lysis of cells with hypotonic solutions is carried out along with some physical (douncing) or enzymatic (lysozyme) method to break open the cells. If one were not to do any of these and just resuspend cells in water, how long would it…
gkadam
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How much energy does human body fat contain?

I have been reading the Wikipedia article about food energy, but as I'm not a biologist, I don't really understand it properly, so I came here to ask you guys. My question is: If a human body needs X kcal of energy and it's taking it from the stored…
Frantisek
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How would one calculate the availability of nucleotides to an enzyme?

How would one calculate the availability of nucleotides to an enzyme like a polymerase ? I imagine an answer in units like nucleotides per second per enzyme, but I'm also imagining an answer that is not a simple rate of reaction. k_on calculations…
Thomas Ingalls
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Why does Rigor Mortis occur after death?

After someone dies they become stiff, this is termed Rigor Mortis and happens because the cells run out of ATP (I think). But why do the cells need it to remain flexible?
Gabriel Fair
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Why is GTP gamma S non hydrolyzable?

GTP gamma S is routinely used in studies of G proteins to stably activate the G protein. Comparing the structures of GTP gamma S and GTP, the Sulphur atom replaces the Oxygen attached to the leaving Phosphate group. However, this is not the bond…
gkadam
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Does drinking coffee have negative effects?

From what I collected, coffee is a magical potion that lets you feel energetic, and essentially not-sleepy. But are there any tradeoffs? I mean, if it was so beneficial, wouldn't the human body produce caffeine naturally?
jcora
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What is the concentration of ATP in an average cell?

Can someone give me some references showing the average concentration of ATP in a cell?
Aditya Chawla
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Where does the proton come in the reduction of NAD?

In our curriculum biology textbook the reduction of NAD+ is depicted as follows: NAD+ + 2 H+ → NADH + H+ If this proton in the products was not present in the reactants, then where does it come from? Alternatively, if it does not take part in the…
Hossein
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Why doesn't life produce diamond, graphene, nanotubes, etc? Could it?

It seems that life is really good at assembling carbon into extremely complicated molecules (e.g. DNA). Graphene is stronger than any materials currently used by biology. Diamond is harder than any biological materials. Do we know about some…
hexagon
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Molecularly, why can you straighten or perm hair?

I'm aware that hair can be curly because of the disulfide bond interactions in between cysteine amino acids in alpha-keratin filaments. However, I'm curious as to the biochemistry involved in straightening hair with a straightening iron, as well as…
LanceLafontaine
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The real definition of carbohydrates

Carbohydrates are often defined as compounds with only C, H, and O and the H and usually the O atoms are in a 2:1 ratio. Exceptions like deoxyribose exist, but why is it recognized as a carbohydrate? What distinguishes a carbohydrate from other…
hello all
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How to prevent protein precipitation?

I'm trying to label a protein with fluorescent dye (TMR succinimidyl ester), but having trouble getting the protocol to work. The protein seems to be stable in distilled water at a the recommended concentration of about 2mg/ml (determined by OD280…
so12311
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What makes a wet dog so stinky?

Similarly, what makes a wet down comforter so stinky? Why do they make no smell except when liquid is added?
Tom
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Is it possible to derive the Michaelis-Menten equation under conditions where the product formation is reversible

Text books etc generally derive the Michaelis-Menten equation for the irreversible case i.e $$\ce{E + S <=> ES -> E +P}$$ I can't see how to do it for the reversible case i.e $$\ce{E + S <=> ES <=> E +P}\;$$ Is it possible?
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