For 1, this is a derivation with Natural Deduction.
Consider a first-order language with a binary predicate $E(x,y)$ :
1) $(∃y)(∀x)(E(x,y) ↔ ¬E(x,x))$ --- assumed [b]
2) $(∀x)(E(x,s) ↔ ¬E(x,x))$ --- assumed [a] for $\exists$-elimination
3) $E(s,s) ↔ ¬E(s,s)$ --- from 2) by $\forall$-elimination
The formula in 3) is a contradiction, since $E(s,s) ↔ E(s,s)$. Thus, we have :
4) $\bot$ --- from 3)
5) $\bot$ --- from 4) by $\exists$-elimination, discharging [a] : $s$ does not occur in 5)
6) $(∃y)(∀x)(E(x,y) ↔ ¬E(x,x)) \to \bot$ --- from 1) and 5) by $\to$-introduction, discharging [a]
$\vdash \lnot (∃y)(∀x)(E(x,y) ↔ ¬E(x,x))$ --- from 6) by abbreviation : $\lnot p := p \to \bot$.
We have used no $\mathsf {ZFC}$ axioms.
Ref to :
Regarding 2, this formula is not valid.
Consider again the formula of first-order language with the binary predicate $E(x,y)$ :
$∀y∃x\lnot E(x,y)$.
If we interpret it into the domain $\mathbb N$ of natural numbers with $E$ interpreted as $\ge$, we have :
$∀y∃x(x < y)$
that is clearly not satisfied for $y=0$.
Thus, being not valid, it is not provable by rules of logic alone, i.e. :
$\nvdash ∀y∃x\lnot E(x,y)$.
In presence of the Axiom Schema of Separation, we can use the Russell's paradox argument to prove that the assumption :
$\exists y \forall x(x \in y)$
produces a contradiction. Simplifying a little :
1) $\exists y \forall x(x \in y)$ --- assumed [a]
2) $\forall x(x \in V)$ --- assumed [b] for $\exists$-elimination
3) $x \in V$ --- from 3) by $\forall$-elimination
4) $\mathsf {ZFC} \vdash \exists z \forall x (x \in z \leftrightarrow x \in V \land x \notin x)$ --- Axiom of Separation
5) $(x \in z \leftrightarrow x \in V \land x \notin x)$ --- from 4), assuming [c] : $\forall x (x \in z \leftrightarrow x \in V \land x \notin x)$ for $\exists$-introduction and by $\forall$-elimination
6) $(x \in z \leftrightarrow x \notin x)$ --- from 3) and 5) by tautological implication
7) $\exists z \forall x (x \in z \leftrightarrow x \notin x)$ --- from 6) by $\forall$-introduction : $x$ not free in assumptions [a], [b] or [c] and from 4) and 5) by $\exists$-elimination : $z$ not free in 7), discharging [c]
8) $\vdash \lnot \exists z \forall x (x \in z \leftrightarrow x \notin x)$ --- proved above
9) $\bot$ --- from 7) and 8)
10) $\bot$ --- from 1) and 2) by $\exists$-elimination : $V$ not free in 10), discharging [b]
11) $\exists y \forall x(x \in y) \to \bot$ --- from 1) and 10) by $\to$-introduction, discharging [a]
$\mathsf {ZFC} \vdash \lnot \exists y \forall x(x \in y)$ --- from 11) by abbreviation.
Thus, we have concluded with :
$\mathsf {ZFC} \vdash\forall y \exists x(x \notin y)$.