Is the following statement true or false?
Let $\{A_i,\ i\in I\}$ be a family of at most countable sets. Suppose the family is totally ordered under set inclusion, i.e. $\forall i\in I,\ j\in I$, either $A_i\subset A_j$ or conversely. Then $\bigcup_{i\in I}A_i$ is also at most countable.
I had this problem when I was trying to prove something else using Zorn's lemma. Although my original problem had a simpler solution, I am now more interested in this new question I came up with, so I won't post my original problem as it is sort of irrelevant.
The difficulty is that $I$ may not be a countable index set. Yet, I was unable to find a counterexample or a proof off the top of my head. Any ideas?