The theory of the Durand-Kerner method provides bounds on roots, based on the Gershgorin circle theorem and more refined bounds based on more complicated methods.
If $z_1,\dots,z_n$ are distinct approximations of the roots, and
$$w_i=-\frac{p(z_i)}{\prod_{k\ne i}(z_k-z_i)},$$
then the roots can be found in the union of the disks $D(z_i+w_i,(n-1)|w_i|)\subset D(z_i,n|w_i|)$. See the literature cited in the wikipedia article for closer bounds and conditions on root disk separation.
Here for the reduced polynomials $p_\pm(z)=(z-1)...(z-n)\pm i\sqrt{n+1}$, $z_k=k$ and $p_\pm(z_k)=\pm i\sqrt{n+1}$, so that $P_n(z)=p_+(z)p_-(z)$, the Weierstrass increments for each factor $p_\pm(z)$ separately can be bounded by
$$|w_i|\le\frac{\sqrt{n+1}}{\lfloor\tfrac n2\rfloor!\,\lceil\tfrac n2\rceil!}$$
which results in a bound
$$|z_{\pm,k}^*-k|\le \frac{n\sqrt{n+1}}{\lfloor\tfrac n2\rfloor!\,\lceil\tfrac n2\rceil!}$$
for the roots $z_{\pm,k}^*$ of $P_n$ independent of the factor in which they occur. This bound is perhaps not as nice for small $n$ as the bound in the conjecture, but in a similar structure and should be better for large $n$ due to the smaller power in the numerator.
If one uses these root bounds, one gets for $n\ge6$ clearly separated roots of $p_\pm$, that is, the claim of the Gershgorin theorem (and earlier Weierstrass) of exactly one root per disk holds.
This is usually demonstrated with a homotopy argument for $p_\pm(t,z)=(z-1)...(z-n)\pm it\sqrt{n-1}$ in that for $t=0$, the integers are the exact roots, and for growing $t$ there is exactly one root path starting from each integer per the implicit function theorem. As long as the root bounding disks, that also grow with factor $t$, stay separate until $t=1$, no root path can change disks.