A solution would be to define a macro that invokes \lstinputlisting three times (for the part before the line to emphasize, the line itself, and the part after the line to emphasize) and adopts the listing style with \lstset to achive the desired highlighting:
\documentclass{article}
\usepackage[T1]{fontenc}
\usepackage[utf8]{inputenc}
\usepackage{listings}
\usepackage{xcolor} % for coloring as emph
\usepackage{etoolbox} % for \ifnumcomp
\usepackage{beramono} % a tt font that has a boldface series
\lstset{basicstyle=\ttfamily}
% The style to be applied for the highlighted line:
\lstdefinestyle{highlight}{basicstyle=\ttfamily\bfseries\color{red}}
%\lstdefinestyle{highlight}{backgroundcolor=\color{orange!30}}
% input listing and emphasize a specific line
% \lstinputemph[listings options]{line number}{filename}
\newcommand{\lstinputemph}[3][\empty]{%
\lstset{aboveskip=0pt, belowskip=0pt, showlines=true,#1}%
\ifnumcomp{#2}{=}{0}{\lstinputlisting{#3}}{%
\ifnumcomp{#2}{=}{1}{}{\lstinputlisting[lastline=\the\numexpr#2-1\relax]{#3}}%
\lstinputlisting[firstnumber={#2},firstline={#2},lastline={#2},style=highlight]{#3}
\lstinputlisting[firstnumber={\the\numexpr#2+1},firstline=\the\numexpr#2+1\relax]{#3}
}% else
}
\begin{document}
\lstinputemph[language=C]{3}{hello.c}
\end{document}
With file hello.c as follows
#include <stdio.h>
void main() {
printf("Hello, World\n")
}
This lead to the following output (second example uses a different background color for the highlighting effect)

