Using
\AtEndDocument{%
\bibliographystyle{alpha}
\bibliography{biblio}
}
will make the subfiles missing the biblio.bib file, because for them the correct path is ../biblio. With bibtex the command \bibliography{biblio} points to a single file, you can not add multiple search paths.
(Using instead \bibliography{../biblio} will succeed on chapters but then it will fail when compiling main.tex)
One way to avoid this is to detect if you are in the preamble or in the text of the document.
This is done with the command \insubfile (from \renewcommand only in subordinated file with subfiles package)
Or see my update at the end!
While compiling the subfiles \bibliography{../References/biblio} will be executed; instead these command(s) will be ignored compiling main.tex, but \bibliography{./References/biblio} will be executed at the end of the document.
I choose a slightly more complex directory arrangement to test the solution. I like to have all the files belonging to a project under the same roof, so to speak.

After compiling each chapter separately, these are the individual results:
01_chapter_intro.tex

02_chapter_one.tex

For main. tex you get

File MyProject/main.tex
%% MyProject/main.tex
\RequirePackage{fix-cm} % Correct scaling of CM font
\documentclass[a4paper,12pt]{memoir}
\usepackage{subfiles}
%% https://tex.stackexchange.com/a/515672/161015
%% https://tex.stackexchange.com/a/16298/161015
\makeatletter
\newcommand{\insubfile}[1]{\ifx@onlypreamble@notprerr\else#1\fi}
\makeatother
\bibliographystyle{alpha}
\begin{document}
\chapter{Thanks}
This is my main document. Starts with \cite{Mer89_WhatWrongThis}.
\subfile{./Chapters/01_chapter_intro}
\subfile{./Chapters/02_chapter_one}
\bibliography{./References/biblio}
\end{document}
File MyProject/Chapters/01_chapter_intro.tex
%% MyProject/Chapters/01_chapter_intro.tex
\documentclass[../main]{subfiles}
\insubfile{\AtEndDocument{\bibliography{../References/biblio}}}
\begin{document}
\chapter{Hello}
And then \cite{einstein}.
\end{document}
File MyProject/Chapters/02_chapter_one.tex
%% MyProject/Chapters/02_chapter_one.tex
\documentclass[../main]{subfiles}
\insubfile{\AtEndDocument{\bibliography{../References/biblio}}}
\begin{document}
\chapter{One}
Only \cite{dirac}.
\end{document}
File MyProject/References/biblio.bib
%%% MyProject/References/biblio.bib
@article{Mer89_WhatWrongThis,
title = {What's {{Wrong}} with This {{Pillow}}?},
author = {Mermin, N. David},
year = {1989},
month = apr,
volume = {42},
pages = {9--11},
publisher = {{American Institute of Physics}},
issn = {0031-9228},
doi = {10.1063/1.2810963},
file = {/home/leo/Zotero/storage/WV73TT5N/1.html},
journal = {Physics Today},
number = {4}
}
@article{einstein,
author={Albert Einstein},
title={Zur Elektrodynamik bewegter Korper},
journal={Annalen der Physik},
volume={322},
number={10},
pages={891--921},
year={1905},
DOI ={http://dx.doi.org/10.1002/andp.19053221004}
}
@book{dirac,
title={The Principles of Quantum Mechanics},
author={Paul Adrien Maurice Dirac},
isbn={9780198520115},
series={International series of monographs on physics},
year={1981},
publisher={Clarendon Press},
keywords = {physics}
}
UPDATE (well ...)
Reading the package subfile document I found out that a new command was added to the class in October 2020.
3.3 Conditional execution of commands
The command \ifSubfilesClassLoaded is useful to execute commands
conditionally, depending on whether the main file is typeset or a
subfile.
\ifSubfilesClassLoaded{% then branch
. . . commands executed when the subfile is typeset . . .
}{% else branch.
. . commands executed when the main file is typeset . . .
}
As an example, this can be used to add the bibliography to the main
document or to the subdocument, whichever is typeset:
ifSubfilesClassLoaded, but I wanted to avoid to hardcode the path depending on whether I'm in the subfile or in the main file, because this solution will not work if my chapters are not all in the same subfolders. I thought that subfix was supposed to solve this issue, and that bibliography was automatically applying the fix on the fly... but apparently it's not working. Your proposed fix is good enough for my needs right now, but anyway I filled a bug there https://github.com/gsalzer/subfiles/issues/29 – tobiasBora Jul 12 '21 at 07:43../../../Referencesin the same way for all chapters. – Simon Dispa Jul 12 '21 at 19:22