The BibTeX field file is not support by the standard BibLaTeX styles. A style supporting it is the reading one.
To use it in other styles, the first thing is to provide formatting instructions for it:
\DeclareFieldFormat{file}{\bibstring{file}\addcolon\space \url{#1}}
The second thing to do is to give instructions to print the field (and at the right position), this means to redefine some existing bib macro to include the field in it. To print it at the end of an entry a possibility is to redefine the finentry bib macro.
\renewbibmacro{finentry}{%
\finentry\addspace
\printfield{file}%
}
Another possibility would be to include the information in the block to print URL or DOI.
\newbibmacro*{doi+eprint+url}{%
\iftoggle{bbx:doi}
{\printfield{doi}}
{}%
\newunit\newblock
\iftoggle{bbx:eprint}
{\usebibmacro{eprint}}
{}%
\newunit\newblock
\iftoggle{bbx:url}
{\usebibmacro{url+urldate}}
{}
\newunit\newblock
\printfield{file}
}
The code above could be refined to include a option (file) in the same way as the option for doi, url and eprint, namely:
\newbool{bbx:file}
to be activated by \usepackage[file,...]{biblatex}, and the last line of the code above can be replaced by
\iftoggle{bbx:file}
{\printfield{file}}
{}
EDIT Solution using \AtEveryBibitem:
\AtEveryBibitem executes the code in its argument immediately after the item code. Thus using \printfield{file} would result that the file name will be printed before the content of the entry. However, one can append it at the end of the content of the driver. The content of the driver is available with the control sequence blx@bbx@<entrytype>. The command \thefield{entrytype} will give us the string needed. Thus, to print the file name at the end of a bib item, we can use the following instruction:
\AtEveryBibitem{\csappto{blx@bbx@\thefield{entrytype}}{\addspace\printfield{file}}}
fileis already defined in BibLaTeX, but it is not used in the vast majority of existing styles. Indeed it is possible to use the map facilities of biber to map it to something used by an existing style. – Guido Nov 10 '12 at 07:57