Note: I take a very different view of the original question in the following discussions and solutions. And, no, I do not like the idea of making + and/or - active in math mode just for alignment purposes.
This problem exists not only for Computer Modern/Latin Modern, and not only for the + and - pair
With the default Computer Modern family (or the Latin Modern family), the width difference problem is barely visible. However, with other math fonts such as newtxmath, newpxmath and mtpro2, this problem is much more visible and it goes beyond the + and - pair. The following MWE illustrates this point:
\documentclass{article}
\usepackage{mathtools}
% Latin Modern
%\usepackage{lmodern}
% newtxmath
\usepackage{newtxtext}
\usepackage{newtxmath}
% mtpro2
%\usepackage{newtxtext}
%\usepackage[lite]{mtpro2}
\begin{document}
Cases: $f(x) = \begin{cases}
+1 & x>0 \\
-1 & x=0 \\
-1 & x\le0
\end{cases}$
Stacked subscripts: $\displaystyle \sum_{\substack{i=0 \\ i>0}}$
\end{document}

We may now conclude that the width differences are by design. More importantly, the behavior is unpredictable across different math fonts and across different design/optical sizes. Clearly, any solutions that involve active +, -, = and so on would be ineffective and dangerous.
How can we get mono-width symbols?
Instead of making characters active (and possibly breaking other things), I propose two specially designed commands:
\tabularrel{<arg>}
and
\tabularbin{<arg>}
The names are inspired by “proportional versus tabular figures”. Each of the two commands essentially makes a box of fixed width according to some “standard symbol”, then it puts the actual symbol horizontally centered. Note that \mathpalette is needed to get the correct math style. Of course, this method of aligning tabular-like math can be applied to any math fonts.
Here is a MWE:
\documentclass{article}
\usepackage{mathtools}
% Latin Modern
%\usepackage{lmodern}
% newtxmath
\usepackage{newtxtext}
\usepackage{newtxmath}
% mtpro2
%\usepackage{newtxtext}
%\usepackage[lite]{mtpro2}
% Standard relation and binary symbols
\newcommand*\standardrel{<}% Change this to `=' for mtpro2
\newcommand*\standardbin{+}
% Tabular relation and binary symbols
\makeatletter
\newcommand*\tabularrel[1]{%
\mathrel{\mathpalette{\@tabularsym\standardrel}{#1}}%
}
\newcommand*\tabularbin[1]{%
\mathbin{\mathpalette{\@tabularsym\standardbin}{#1}}%
}
\newcommand*\@tabularsym[3]{%
% #1: standard symbol
% #2: math style
% #3: user symbol
\setbox\z@\hbox{$#2#1\m@th$}%
\hbox to\wd\z@{\hss$#2#3\m@th$\hss}%
}
\makeatother
\begin{document}
Cases: $f(x) = \begin{cases}
\tabularbin+1 & x\tabularrel>0 \\
\tabularbin-1 & x\tabularrel=0 \\
\tabularbin-1 & x\tabularrel\le0
\end{cases}$
Stacked subscripts: $\displaystyle \sum_{\substack{i\tabularrel=0 \\ i\tabularrel>0}}$
\end{document}
Voilà!!!

For most “one-liner” equations, there are no need for mono-width symbols. However, when typing inside tabular-like environments, e.g., tables, arrays, cases, matrices, etc., horizontal alignment may be crucial.
cmr12, which has a slightly narrowed aspect ratio, and the minus is scaled fromcmsy10, sincecmsy12doesn't exist. but i am surprised that the 6pt widths are different, since bothcmr6andcmsy6were among the "original 75" fonts defined with metafont. i will investigate further when time permits. – barbara beeton Apr 10 '17 at 21:19-which does not possess the same width as+, or having a-that does not work in superscripts? Is there any way to define a new command which exhibits\mathbinspacing and simultaneously works when used as a superscript without having to be enclosed in parentheses? – wea0 Jul 29 '17 at 00:13