It sort of looks like you're trying to use the dateplot library, but as described in the manual, you need to specify a date, not just the time:

Note also that it should be date coordinates in=x, not Time coordinates in=x. After adding a date to the input file, and adjusting the values for xmin/xmax accordingly:
\begin{filecontents}{mwe.csv}
Time,a,b
2020-1-1 00:00,0,10
2020-1-1 10:30,6,4
2020-1-1 11:30,5,5
2020-1-1 12:30,3,7
2020-1-1 13:30,2,8
\end{filecontents}
\documentclass[border=5mm]{standalone}
\usepackage{pgfplots}
\usepgfplotslibrary{dateplot}
\begin{document}
\begin{tikzpicture}
\begin{axis}[
date coordinates in=x,
xticklabel={\hour.\minute},
x tick label style={align=center,rotate=45},
ymajorgrids,
xmin={2020-1-1 00:00},
xmax={2020-1-1 21:30},
legend pos=north west
]
\addplot [ycomb,color=blue] table [col sep=comma,y=a] {mwe.csv};
\addlegendentry{a}
\addplot [ycomb,color=red] table [col sep=comma,y=b] {mwe.csv};
\addlegendentry{b}
\end{axis}
\end{tikzpicture}
\end{document}

Addendum
If, on the other hand, you're trying to use Symbol 1's answer at How to create a date and time plot with TikZ and pgfplots then see the code below. It would have been great if you had mentioned this in the question (though I suppose I could have waited until you supplied more information, as requested by frougon ...).
You still need to make sure you use the right name for the style, Symbol 1's code defines time coordinates in, you used Time coordinates in. This is case sensitive, so you need to use the same case in both style definition and use.
I do note that the legend is empty. Stefan Pinnow demonstrates how to get a more useful legend for ycomb plots in https://tex.stackexchange.com/a/357469/.
\begin{filecontents*}{mwe.csv}
Time,a,b
00:00,0,10
10:30,6,4
11:30,5,5
12:30,3,7
13:30,2,8
\end{filecontents*}
\documentclass[border=5mm]{standalone}
\usepackage{pgfplots}
\usepgfplotslibrary{dateplot}
% the following is from https://tex.stackexchange.com/a/288225/
\def\pgfplotslibdateplothour:minutetofloat#1:#2.{
\pgfmathparse{#1+#2/60}
}
\def\pgfplotslibdateplofloattothour:minute#1.{
\pgfmathsetmacro\hour{int(floor(#1))}
\pgfmathsetmacro\minute{int((#1-\hour)*60)}
\ifnum\hour<10\edef\hour{0\hour}\fi
\ifnum\minute<10\edef\minute{0\minute}\fi
}
\pgfplotsset{
/pgfplots/time coordinates in/.code={%
\pgfkeysdef{/pgfplots/#1 coord trafo}{%
\pgfplotslibdateplothour:minutetofloat##1.
}
\pgfkeysdef{/pgfplots/#1 coord inv trafo}{
\pgfplotslibdateplofloattothour:minute##1.
}
}
}
\begin{document}
\begin{tikzpicture}
\begin{axis}[
time coordinates in=x,
xticklabel={\hour.\minute},
x tick label style={align=center,rotate=45},
ymajorgrids,
xmin={00:00},
xmax={21:30},
legend pos=north west,
]
\addplot [ycomb, draw=blue] table [col sep=comma,trim cells=true,y=a] {mwe.csv};
\addlegendentry{a}
\addplot [ycomb,color=red] table [col sep=comma,trim cells=true,y=b] {mwe.csv};
\addlegendentry{b}
\end{axis}
\end{tikzpicture}
\end{document}
\documentclass, you don't need\usepackage{filecontents}with recent LaTeX, you are missing other packages though...). Thank you. – frougon Mar 27 '20 at 11:35