The definition of \obeylines is
% In \obeylines, we say `\let^^M=\par' instead of `\def^^M{\par}'
% since this allows, for example, `\let\par=\cr \obeylines \halign{...'
{\catcode`\^^M=\active % these lines must end with %
\gdef\obeylines{\catcode`\^^M\active \let^^M\par}%
\global\let^^M\par} % this is in case ^^M appears in a \write
So you can try:
\def\disobeylines{\catcode`\^^M=5 }
The example:
\def\disobeylines{\catcode`\^^M=5 }
\obeylines
We obey, and end up on separate lines.
We obey.
\disobeylines
We disobey, and end up on the same line.
We disobey.
\bye
EDIT
As egreg pointed out in his comment, Donald Knuth doesn't provide any \disobeylines. In the TeXBook at page 94 the command \obeylines is introduced with the following paragraph:
You may have several consecutive lines of input for which you want the
output to appear line-for-line in the same way. One solution is to
type \par at the end of each input line; but that’s somewhat of a
nuisance, so plain TeX provides the abbreviation \obeylines, which
causes each end-of-line in the input to be like \par. After you say
\obeylines you will get one line of output per line of input, unless
an input line ends with % or unless it is so long that it must be
broken. For example, you probably want to use \obeylines if you are
typesetting a poem. Be sure to enclose \obeylines in a group,
unless you want this "poetry mode" to continue to the end of your
document.