I suspect some kind of bug when inscribing a circle into an equilateral triangle. It works perfectly with many other triangles, but when you approach too near 60°, it fails to do it. For example, with an angle of 59.9°:
\documentclass{article}
\usepackage{tkz-euclide}
\begin{document}
\begin{tikzpicture}[scale=1]
\coordinate (A) at (0,0);
\coordinate (B) at (4,0);
\coordinate (C) at (59.9:4);
\tkzDefCircle[in](A,B,C)
\tkzGetPoint{O} \tkzGetLength{rIN}
\tkzDrawPoints(A,B,C,O)
\tkzDrawCircle[R,blue](O,\rIN pt)
\tkzLabelPoints(A,B,C,O)
\tkzDrawPolygon(A,B,C)
\end{tikzpicture}
\end{document}

But with an angle of 59.99°, it fails:

So you're not doing anything wrong. If you want to draw it anyway with tkz-euclide, you can do it "by hand":
\documentclass{article}
\usepackage{tkz-euclide}
\begin{document}
\begin{tikzpicture}[scale=1]
\coordinate (A) at (0,0);
\coordinate (B) at (4,0);
\coordinate (C) at (60:4);
\tkzDefBarycentricPoint(A=1,B=1,C=1) \tkzGetPoint{O}
\tkzInterLL(A,O)(C,B) \tkzGetPoint{A'}
\tkzDrawCircle[color=blue](O,A')
\tkzDrawPoints(A,B,C,O)
\tkzLabelPoints(A,B,C,O)
\tkzDrawPolygon(A,B,C)
\end{tikzpicture}
\end{document}