For your edit, the preferred way to change the bibliography font size to \small is by using \renewcommand*{\bibfont}{\small} (courtesy of moewe). There is probably a better way to change the linespacing of individual entries within biblatex but the following code does as you require (assuming you are using the title option of \printbibliography):
\documentclass[oneside]{book}
\usepackage[backend=biber,natbib=true,style=numeric]{biblatex}
\usepackage{blindtext}
\usepackage{setspace}
\begin{filecontents}{\jobname.bib}
@article{one,
author = {Chi, Yu-Chou and Lee, Shou-Lun and Lai, Ching-Long and Lee, Yung-Pin and Lee, Shiao-Pieng and Chiang, Chien-Ping and Yin, Shih-Jiun},
journal = {Chemico-Biological Interactions},
pages = {134--141},
title = {{Ethanol oxidation and the inhibition by drugs in human liver, stomach and small intestine: Quantitative assessment with numerical organ modeling of alcohol dehydrogenase isozymes}},
volume = {258},
year = {2016}
}
@article{two,
author = {Adolf-Bryfogle, Jared and Teets, Frank D and Bahl, Christopher D},
journal = {Current Opinion in Structural Biology},
pages = {170--177},
title = {{Toward complete rational control over protein structure and function through computational design}},
volume = {66},
year = {2021}
}
\end{filecontents}
\addbibresource{\jobname.bib}
\renewcommand*{\bibfont}{\small} % Answer to edit
\onehalfspacing
\begin{document}
\chapter{One}
\blindtext \cite{one, two}
% Answer to main question
\begingroup
\singlespacing
\printbibliography[title={\onehalfspacing Really long title so that it spans two lines so the line stretching can be observed}]
\endgroup
\end{document}
This example produces this in the main text:

And produces this in the bibliography:

I have used \begin{filecontents}{\jobname.bib}...\end{filecontents} to include references for examples and you do not require it, the package blindtext has been used to create filler text in chapter 1 and you can change \addbibresource to your reference file. Using \begingroup and \endgroup keeps everything which occurs inside local. Hope this helps.