I can think of two suggestions:
Use \dfrac instead of \frac (while eliminating the unnecessary parentheses) for the second additive term in the numerator.
Replace the repeated term \sqrt{\pi^{2}r^{10}+9V^{2}r^{4}} with a symbol, say, W.
I dare say the second expression is a bit more readable.
Either way, do please also supply the missing denominator term.

\documentclass{article}
\usepackage{amsmath}
\newcommand\dv[3][]{\frac{\mathrm{d}^{#1}\mathit{#2}}{\mathrm{d}#3^{#1}}}
\begin{document}
Set $W\equiv{(\pi^{2}r^{10}+9V^{2}r^{4})}^{1/2}$. Then
\begin{align}
\dv[2]{SA}{r}
&=\frac{{({\pi^{2}r^{10}+9V^{2}r^{4}})^{\frac{1}{2}}}(12\pi^{2}r^{5})-
%%\left({
\dfrac{2\pi^{2}r^{6}-9V^{2}}{2\sqrt{\pi^{2}r^{10}+9V^{2}r^{4}}}
%%}\right)
}{\text{(missing denominator??)}} \[2\jot]
&= \frac{12\pi^{2}r^{5}W-(\pi^{2}r^{6}-4.5V^{2})/W%
}{\text{(missing denominator??)}}
\end{align}
\end{document}
align*come from in your output??? why is there no denominator to the fraction? – David Carlisle Nov 30 '21 at 19:26