Here is a possible approach that redefines the titlepage template in order to insert two authors.
Example:
\documentclass{beamer}
\usepackage{lmodern}
%-------------------------------------------------
% title page with two authors
\makeatletter
\setbeamertemplate{title page}{
\centering
\begin{beamercolorbox}[rounded=true,shadow=true,sep=8pt,center]{title}
\usebeamerfont{title}\inserttitle\par%
\ifx\insertsubtitle\@empty%
\else%
\vskip0.25em%
{\usebeamerfont{subtitle}\usebeamercolor[fg]{subtitle}\insertsubtitle\par}%
\fi%
\end{beamercolorbox}
\vfill
\begin{beamercolorbox}{author}
\begin{columns}[T]
\column{.28\textwidth}%
\centering
\usebeamerfont{author}John Doe\\
john@example.com
\column{.25\textwidth}%
\centering
\usebeamerfont{author}Jane Doe\\
jane@example.com
\end{columns}
\end{beamercolorbox}
\vfill
\usebeamerfont{institute}\insertinstitute \par
\vfill
\centering
\usebeamerfont{date}\insertdate\par
\vfill
\begin{beamercolorbox}[center]{titlegraphic}
\inserttitlegraphic
\end{beamercolorbox}
}
\makeatother
\title{My title}
\subtitle{my subtitle}
\institute{My institute}
\begin{document}
\begin{frame}
\titlepage
\end{frame}
\end{document}
Authors are inserted by means of the columns environment and the result is:

Of course this holds regardless the theme chosen and it is possible to extend it: three authors, three columns and so on.