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I have a series of 37 b/w microscopy photos. The whole sample shifts slightly (by ~10 pixels) from photo to photo (systematic error). See an example photo here and download two photos pos4_0.dat and pos4_40.dat, shifted by ~10px. Moreover, there are major intensity variations. Nevertheless, the main structure is always visible.

photo

I want to track the trajectory of some points through the photo series. Later on, I will stabilize my regions of interest. The last example in Documentation would be excactly what I need, but ImageFeatureTrack doesn't work.

import = Import[#] & /@ {"pos4_0.dat", "pos4_40.dat"}
images = Image[Rescale[#]] & /@ import
ImageFeatureTrack[images]
Out[55]={{}, {}}

How can I improve the performance of ImageFeatureTrack or solve my problem in another way?

Thanks.

keyx
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    Can you post the images? – M.R. Sep 30 '15 at 14:21
  • You could try helping it out by giving it the coordinates of the feature in the first image. It looks like it's not finding a feature to begin with. – N.J.Evans Sep 30 '15 at 14:23
  • @M.R. raw data is given as download link in the first paragraph. Does this work for you? – keyx Sep 30 '15 at 14:27
  • Your Images are 8 MB each. Perhaps you could reduce the resolution for this question ... – Dr. belisarius Sep 30 '15 at 14:36
  • @N.J.Evans: I didn't succeed with that. Since the photos are gray-in-gray, no single pixel seems to be outstanding. I would like to define a whole region as feature. – keyx Sep 30 '15 at 14:38
  • Similar still unanswered but with some suggestions. One Solution but operates on WRI's example data, which the image processing functions usually seem to be tailored to. An alternative to ImageFeatureTracking. – IPoiler Sep 30 '15 at 15:59
  • For some reason the download does not work for me. Anyhow, looking at the samples in the question I would think that FindGeometricTransform or ImageAlign are better suited to this problem. – Matthias Odisio Oct 01 '15 at 15:42
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    I'm voting to close this question as off-topic because the sample images linked in the question aren't available for download and the question is not answerable without them – Dr. belisarius Feb 12 '16 at 14:17

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